PREPARATION OF MATHANTHAILAM MEDIATED ZNONPS AND IT'S ANTIMICROBIAL, ANTI-INFLAMMATORY ACTIVITY AND CELL VIABILITY USING MTT ASSAY

Abstract

Zinc oxide nanoparticles (ZnONPs), mediated by Maththan Oil, were rigorously evaluated for their antimicrobial and anti-inflammatory potentials, alongside an assessment of cytotoxicity, across several bioassays. The research specifically targeted the antibacterial efficacy of ZnONPs against diverse bacterial strains such as Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, and Pseudomonas sp., employing the agar well diffusion method. A dose-dependent escalation in the zones of inhibition was meticulously recorded, signifying robust antibacterial action; the zones ranged from 20 mm to a substantial 40 mm as concentrations of Maththan Oil were incrementally increased from 25 µg/mL to 100 µg/mL.

Concurrently, the anti-inflammatory properties of these nanoparticles were quantified through the inhibition of Bovine Serum Albumin (BSA) denaturation. The results illuminated a pronounced dose-dependent anti-inflammatory activity, with up to 75% inhibition of protein denaturation at a concentration of 50 µg/mL, highlighting the nanoparticles' significant potential for pharmaceutical applications where modulation of inflammatory responses is paramount.

Further explorations into the cytotoxic impacts of MaththanThailam mediated ZnONPs were conducted using an MTT assay on murine fibroblast cells (3T3-L1). This assay delineated a clear dose-dependent cytotoxicity, where cell viability was moderately high at lower concentrations but exhibited a marked reduction at higher dosages, declining to 69% at 100 µg/mL.

These comprehensive evaluations not only underscore the dual functionality of Maththan Oil mediated ZnONPs exemplified by potent antibacterial and anti-inflammatory activities but also delineate their cytotoxic profile at elevated concentrations, warranting further investigative and clinical studies to elucidate their mechanisms of action and potential therapeutic applications. This study provides a foundational impetus for subsequent translational research aimed at harnessing the therapeutic efficacies of ZnONPs in managing bacterial infections and inflammatory conditions within clinical settings.