PHYTOCHEMICAL SCREENING AND IN-VITRO ANTI- INFLAMMATORY ACTIVITY OF AQUEOUS ETHANOLIC EXTRACT OF DRIED LEAVES OF CROTALARIA VERRUCOSA

Abstract

The main aim of this study was to determine the pharmacognostic, phytochemical parameters and to determine the anti-inflammatory activity of the aqueous ethanolic extract of Crotalaria verrucosa leaves. The aqueous ethanolic plant extracts of leaves of Crotalaria verrucosa were prepared using the standard maceration method. Then conducted phytochemical screening by various pharmacognostic tests and in-vitro anti-inflammatory activity by protein denaturation method. The preliminary phytochemical analysis revealed that the aq. ethanolic leaf extract of Crotalaria verrucosa were shown positive results towards to carbohydrates, glycosides, phenols, flavonoids, proteins and amino acids and revealed the ethanolic extract showed the positive results towards carbohydrates, glycosides, phenols, proteins and amino acids. We conducted and reported the Inhibitory concentration (IC₅₀) of anti-inflammatory activity of Crotalaria verrucosa leaves hydro -alcoholic extract was found at concentration of 240 µg/mL.