EVALUATING THE CYTOTOXIC IMPACT OF A METHANOLIC EXTRACT OF CASSIA AURICULATA COMBINED WITH ALPHA-TOCOPHEROL AND POVIDONE-IODINE IN A HERBAL-BASED WOUND DRESSING ON A FIBROBLAST CELL LINE

Abstract

Background: Wound management often requires treatments that promote healing while minimizing cytotoxicity. This study evaluates the cytotoxic effects and potential therapeutic benefits of a herbal-based wound dressing formulated with a methanolic extract of Cassia auriculata, alpha-tocopherol, and povidone-iodine using a fibroblast cell line.

Materials and Methods: The cytotoxicity of the herbal-based wound dressing was assessed using an in vitro fibroblast cell viability assay. Concentrations ranging from 5 µg/mL to 80 µg/mL were tested to evaluate the formulation's impact on cell survival over two consecutive days, with a control group maintained at 100% viability for comparison.

Results: Results indicated high fibroblast survival rates across all tested concentrations, with over 90% viability even at the highest concentration (80 µg/mL). The formulation demonstrated minimal cytotoxicity, suggesting a high degree of biocompatibility.

Discussion: The combination of Cassia auriculata, known for its antioxidant properties, with alpha-tocopherol and povidone-iodine likely contributed to the formulation's efficacy by providing both microbial protection and oxidative stress reduction. The study aligns with existing literature that supports the use of herbal extracts in enhancing wound healing with minimal adverse effects.

Conclusion: The herbal-based wound dressing exhibits potential as a safe and effective therapeutic option for wound management, promoting fibroblast viability and tissue regeneration. Future studies should focus on in vivo testing and formulation optimization to confirm these findings and extend its clinical applications.